Marita Vedovelli Cardozo
Sao Paulo State University , Brazil
Title: Klebsiella pneumoniae and Escherichia coli producing extended spectrum β-lactamase in broilers production chain
Biography
Biography: Marita Vedovelli Cardozo
Abstract
The extended spectrum β-lactamases (ESBL) are enzymes that hydrolyze the β-lactam ring of penicillins, cephalosporins
and aztreonam, conferring resistance to these antimicrobials. There are an increasing number of infections caused by
Gram-negative bacteria producing extended spectrum β-lactamases and the emergence of new therapeutic resources does
not follow the evolution of resistance mechanisms, making them a serious public health problem. Brazil is the largest exporter
of chicken means that the presence of ESBL and it becomes also a potential risk in the agribusiness sector. Based on this, the
present study aims to characterize ESBL production in Enterobacteriaceae isolated from broilers production chain, including
animals, food and consumers. Of the 300 samples collected from cloacal swab, chicken meat and human feces, 36 were resistant
to cefotaxime (CTX) in antimicrobial phenotypic screening. After, by microarray molecular detection, it was found positive
isolates to CTX-M gene. Each positive CTX-M sample was subjected to PCR to confirm the presence of CTX-M-1 and
CTX-M-2 genes and the sequencing showed that all positive samples from CTX-M-1 group belonged to CTX-M-15 gene and
all positive samples from CTX-M-2 group belonged to CTX-M-2 gene. Moreover, identification by MALDI-TOF showed that
36 isolates, 15 were classified as Klebsiella pneumoniae and 21 isolates belong to Escherichia coli. These results evidence of the
correlation of resistance genes between animals and humans show the need to reduce the use of antibiotics in the production
of broilers in the country to combat the spread of antibiotic resistance.